Author response image 7. Genetic perturbation of calcineurin/NFAT signaling persistently prevented the glucose-responsiveness of β-cells.

(A-B) Numbers of glucose-responsive β-cells (A) and their maximal Ca²⁺ responses to glucose (B) in age-matched control and dn-zCnA-expressing embryos at 48 hpf, 72 hpf and 7 dpf. n = 5-7 embryos per stage. *p < 0.05, **p < 0.01, ***p < 0.001; ns, not significant.