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Biomineralization assay using Alizarin red staining after 7 days and 14 days of cell proliferation and differentiation. (A) Alizarin red staining shows mineralization in extracellular matrix of MC3T3-E1 cells grown on plastic and differently treated Ti surfaces for 7 days and 14 days. (B) High magnifications of Alizarin red staining of MC3T-E1 cells, which were cultured on the differently treated surfaces for 14 days. The inset shows a representative mineralized nodule (arrow).
