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. 2016 Dec 24;12(1):60–69. doi: 10.1016/j.jds.2016.10.003

Figure 6.

Figure 6

Treg and Th17 function-associated cytokines in the spleen and moDCs. Splenic T cells of ApoE–/– mice and ApoE–/––P. gingivalis mice were isolated and stimulated with anti-CD3 and anti-CD28 antibodies for 72 hours. The supernatants were collected. (A) IL-10 (B) and IL-17 in supernatants of T cells were determined by ELISA. (C) IL-6 in supernatants of splenic cells was assessed by ELISA (n = 6 mice per group).(D) Bone marrow cells were isolated from 5–6-week-old ApoE–/– mice, cells were cultured with 20 ng/mL granulocyte-macrophage colony stimulating factor (GM-CSF) and 1 ng/mL IL-4 for 9 days to obtain DCs. DCs were infected with P. gingivalis at multiplicity of infection (MOI) 0, 25 and 100 for 24 hours. Supernatants were collected to detect the production of IL-6. * P < 0.05 vs. ApoE–/– (Figures 6C). $ P < 0.0167 versus con, Bonferroni correction, α = 0.05/3 (Figure 6D). # P < 0.0167 versus 1:25, Bonferroni correction, α = 0.05/3. DC = dendritic cells; ELISA = enzyme-linked immunosorbent assay; IL = interleukin; moDC = mouse dendritic cells; Pg = Porphyromonas gingivalis.