Figure 4.
Gene expression of type I collagen α1, osteomodulin (OMD), alkaline phosphatase (ALP), bone morphogenetc proteins (BMPs), and transforming growth factor (TGF)-β in human dental follicle cells. Human dental follicle cells were cultured in growth medium (GM), osteogenic induction medium (OIM) supplemented with 10% fetal bovine serum (FBS), or OIM supplemented with the indicated PRGFs concentrations for 3 or 7 days. The gene expression was examined with real-time polymerase chain reaction. Values represent the means ± standard deviation of the results from three independent experiments. * P < 0.05. ** P < 0.01. *** P < 0.05 compared with Culture Day 0.