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Diagnosis of a CD19-negative DLBCL. (A) Lymphoma cells in bone marrow smears (MGG staining, ×500 magnification). (B) Flow cytometry analysis of the lymphoma cells (red dots) and residual normal B-cells (blue dots) in the blood, after staining of CD19 and CD20 (left) and kappa and lambda light chains (right). (C) Bone marrow biopsy showing infiltration by the DLBCL (left panel: hematoxylin eosin saffron staining, ×20 magnification, right panel, CD20 staining, ×40 magnification).
