Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Sep 28;104(3):505–515. doi: 10.3324/haematol.2018.192823

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright© 2019 Ferrata Storti Foundation

Material published in Haematologica is covered by copyright. All rights are reserved to the Ferrata Storti Foundation. Use of published material is allowed under the following terms and conditions:

https://creativecommons.org/licenses/by-nc/4.0/legalcode. Copies of published material are allowed for personal or internal use. Sharing published material for non-commercial purposes is subject to the following conditions:

https://creativecommons.org/licenses/by-nc/4.0/legalcode, sect. 3. Reproducing and sharing published material for commercial purposes is not allowed without permission in writing from the publisher.

PMC Copyright notice

Figure 3. — Tissue transglutaminase (TG2) expression drives both expression of NCF2 and GP91PHOX respiratory burst oxidase genes and the generation of reactive oxygen species (ROS). (A and B) Relative mRNA expressions of NCF2 and GP91PHOX upon ATRA and ATRA + NC9 treatment over 11 days were determined at the indicated time points by real-time Q-PCR and normalized to cyclophilin-D mRNA levels in NB4-WT, TG2-C, TG2-KD, TG2-ha, and TG2-KO cells. Results are the mean±Standard Deviation of 3 independent experiments. (C) Production of ROS was determined for each cell line by using a luminescence-based method in triplicates (n=5) and reported as relative light units (RLU). Values are normalized to 100 μg protein of cell lysate content, respectively. Statistical significance was determined via two-way analysis of variance (ANOVA; Bonferroni post-hoc test; *P<0.05, **P<0.01, ***P<0.005 ****P<0.001). GAPDH: glyceraldehyde 3-phosphate dehydrogenase; ATRA: all-trans retinoic acid.