Table 2.

Primers and PCR conditions used for determination of the four VDR SNPs.

SNP name	dbSNP	PCR Primer	PCR condition
SNP 1 TaqI C/T	rs731236	F 5-CAG AGC ATG GAC AGG GAG CAA-3 R 5-GCA ACT CCT CAT GGC TGA GGT CTC-3	Denaturation at 95 °C for 1 min, 35 cycles at 95 °C for 1 min, 66 °C for 1 min, 72 °C for 2 min, final extension at 72 °C for 10 min PCR products were digested with TaqI (5°U/sample) at 65 °C for 4 h
SNP 2 ApaI G/T	rs7975232	F 5-CAG AGC ATG GAC AGG GAG CAA-3 R 5-GCA ACT CCT CAT GGC TGA GGT CTC-3	Denaturation at 95 °C for 1 min, 35 cycles at 95 °C for 1 min, 66 °C for 1 min, 72 °C for 2 min, final extension at 72 °C for 10 min PCR products were digested with ApaI (10°U/sample) at 22 °C for 4 h
SNP 3 BsmI G/A	rs1544410	F 5-CAA CCA AGA CTA CAA GTA CCG CGT CAGTGA-3 R 5-AAC CAG CGG GAA GAG GTC AAG GG-3	Denaturation at 95 °C for 2 min, 35 cycles at 95 °C for 1 min, 63 °C for 1 min, 72 °C for 1.5 min, final extension at 72 °C for 7 min PCR products were digested with 1U BsmI at 65 °C overnight
SNP 4 FokI T/C	rs2228570	F 5-ATG GAA ACA CCT TGC TTC TTC TCC CTC-3 R 5-AGC TGG CCC TGG CAC TGA CTC TGC TCT-3	Denaturation at 95 °C for 3 min, 35 cycles at 94 °C for 30 s, 60 °C for 30 s, 72 °C for 30 s, final extension at 72 °C for 5 min PCR products were digested with FokI at 37 °C for 3 h