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Metformin inhibits cell viability and induces apoptosis in FaDu cells. (A) CCK-8 assay was used to determine the cell viability after 48 h of a serial concentrations of metformin (left) treatment and after 8 mM metformin treatment for a serial of time points (right) in FaDu cells. (B) BrdU assay was used to determine the cell proliferation rate in FaDu cells. (C) Flow cytometry was used to measured cell apoptosis after 8 mM metformin treatment for 48 h in FaDu cells. ∗P < 0.05.
