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. 2019 Feb 22;10:143. doi: 10.3389/fphar.2019.00143

FIGURE 2.

FIGURE 2

Rescue of SNHG7 reverse metformin-mediated inhibitory effects in vitro and in vivo. (A) The heatmap showed the differentially expression lncRNAs in metformin (8 mM for 48 h) treated FaDu cells. SNHG7 was significantly decreased by metformin compared with vehicle treated cells. (B) QPCR was performed to measure the SNHG7, LINC01504, and LINC00189 expression after 48 h of a serial concentrations of metformin treatment in FaDu cells. (C) QPCR was performed to measure the SNHG7, LINC01504, and LINC00189 expression after 8 mM metformin treatment for a serial of time points in FaDu cells. (D) QPCR was performed to measure the SNHG7 expression after SNHG7 lentivirus infection. (E) CCK-8 assay was used to determine the cell viability after metformin treatment (8 mM for 48 h) or combined with SNHG7 lentivirus. (F) Flow cytometry was used to measured cell apoptosis after metformin treatment (8 mM for 48 h) or combined with SNHG7 lentivirus. (G) FaDu cells treated with metformin or together with SNHG7 lentivirus were injected into nude mice. The cells untreated were used as negative control. Tumor volumes were calculated and shown. (H) QPCR was performed to measure the SNHG7 expression in tumor tissues from nude mice. (I) Western blot was performed to measure the p-AMPK and AMPK expression in tumor tissues from nude mice. ∗P < 0.05.