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. 2019 Feb 22;10:146. doi: 10.3389/fphar.2019.00146

Figure 3.

Figure 3

Effect of Ang-1–7 and AVE 0991 on cAMP and Ca2+ levels in MasR-expressing cells. Cells transfected with pcDNA3.1/c-myc-MasR or pcDNA3.1 were incubated with increasing concentrations of Ang-(1–7) (A) or AVE 0991 (B) in presence of 1 mM IBMX and 1 μM FSK. The levels of cAMP were determined after 5 min 100% corresponds to basal levels of cAMP. Data represent mean ± SEM of three independent experiments. Variation of the intracellular Ca2+ concentration in A549 cells in response to Ang-(1–7). A549 cells with endogenous expression of MasR (C,D) or transfected either with the empty pcDNA 3.1 vector (E) or with the pcDNA 3.1/myc-MasR vector (F) were placed in 96-well plates 24 h before the experiment. Cells were incubated with FURA 2-AM, washed and stimulated with Histamine 10-4 M (positive control) and Ang-(1–7) 10-7 M (C–F). Arrows indicate addition of the stimulus. Fluorescence determinations were performed as described in Section “Materials and Methods.” Results are representative of three independent experiments.