Figure 6.

Effects of daily, volitional physical activity on mRNA splicing

A, representative endpoint polymerase chain reaction (EPPCR) gel electrophoresis UV images of mRNA splicing assays for Ca²⁺‐calmodulin‐dependent protein kinase IIβ (CaMKIIβ), sarco/endoplasmic reticulum Ca²⁺‐ATPase (ATP2A1), muscle‐specific voltage‐gated chloride channel (CLC‐1), dihydropyridine receptor (DHPR), ryanodine receptor 1 (RYR1), and Bridging integrator 1 (BIN1) in the GAST muscles from the three experimental groups. Specific exon alternative splicing diagrams are displayed to the right of each corresponding image. Molecular weight (bp) markers appear on the right of gels. B, graphical summaries of the percentage exon inclusion of EPPCR data in panel A. C, graphical summaries of quantitative, real‐time PCR analysis of CaMKIIβ, ATP2A1 and CLC‐1 spliced isoform abundance as indicated relative to the amount of the full‐length (pan) sequence. n = 8; ^* P < 0.05 vs. SED‐WT; ^# P < 0.05 vs. SED‐DM1.