Table I.

Content of cockroach allergen extracts

German cockroach extract		Bla g 1 (μg/mL)	Bla g 2 (μg/mL)	Bla g 5 (μg/mL)	Protein concentration APA (mg/mL)	Bla g 1/Prot conc (μg/mg)	Bla g 2/Prot conc (μg/mg)	Bla g 5/Prot conc (μg/mg)	Three allergens/Prot conc (μg/mg)	Endotoxin LAL (EU/mL)
1	Commercial - WB - Hum	41.81	30.30	0.130	7.00	5.97	4.33	0.02	10.32	1617
2	Commercial - WB - Hum	10.32	2.86	0.020	3.14	3.28	0.91	0.01	4.20	10892
3	Commercial - WB - Hum	7.59	3.13	0.081	2.22	3.42	1.41	0.04	4.87	3175
4	Commercial - WB - Hum	8.06	3.13	0.023	2.46	3.28	1.27	0.01	4.56	13418
5	Commercial - WB - Vet	21.44	0.10	<0.005	1.75	12.27	0.06	0.00	12.33	467
6	Commercial - WB - Vet	9.01	4.88	0.244	2.96	3.04	1.65	0.08	4.77	3069
7	Commercial - WB - Res	13.68	17.52	0.178	3.04	4.50	5.76	0.06	10.32	1889
8	Commercial - WB - Res	22.79	12.68	0.030	4.35	5.24	2.91	0.01	8.16	1546
9*	Commercial - WB - Res	44.60	19.01	0.248	6.22	7.17	3.06	0.04	10.27	13782
10	In-house - Fecal - Res	127.82	72.80	<0.005	7.71	16.58	9.44	0.00	26.02	342622
11	In-house - Fecal - Res	150.13	26.70	<0.005	6.03	24.90	4.43	0.00	29.33	152713
12	In-house - Frass - Res	49.75	22.96	<0.005	1.35	36.85	17.01	0.00	53.86	39934
13**	Negative control	<0.008	<0.01	<0.005	5.39	0.00	0.00	0.00	0.00	1412
Average***		42.25	18.01	0.08	4.02	10.54	4.35	0.02	14.92	48760
Stdev***		47.81	20.11	0.10	2.18	10.65	4.74	0.03	14.74	101932
		20x	728x	12x	6x					733x
		Averages:
	WB	19.92	10.40	0.11	3.68
	Hum	16.95	9.86	0.06	3.71
	Vet	15.23	2.49	0.12	2.36
	Res	27.02	16.40	0.15	4.54
	Fec-Frass	109.23	40.82	0.01	5.03

The lowest and highest values of allergen and endotoxin concentrations are underlined, and the fold difference between them is indicated in the bottom row.

^*Reference extract

^**Negative control (allergen concentrations were lower than the indicated lower limit of detection).

^***Negative control was excluded from the calculation of average and standard deviation (Stdev).