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(A) Representative myobundle cross-sectional images stained for dystrophin (red). (B, C) Quantification of (B) myotube cross-sectional area and (C) myotube diameter. (D) Frequency histogram of myotube diameters (n = 500 myotubes from N = 4 donors per group). *, P<0.001 vs. CTL; # P<0.001 vs. 1Hz.
