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. Author manuscript; available in PMC: 2020 May 21.
Published in final edited form as: Neuroscience. 2018 Sep 1;407:108–119. doi: 10.1016/j.neuroscience.2018.08.023

Figure 1. Cochlear application of ouabain elevates ABR thresholds with minimal effects on DPOAEs.

Figure 1.

(A) A 1mM Ouabain solution or distilled water (vehicle) was applied to the left ear of adult CBA/CaJ mice. Distortion product otoacoustic emissions (DPOAEs) and auditory brainstem responses (ABRs) were measured 30 days later, just before processing tissue from the contralateral inferior colliculus (IC) and auditory cortex (ACtx) for in situ hybridization. (B) DPOAE thresholds from ouabain- and vehicle-treated ears (blue and black, respectively). Inset: DPOAE emission amplitude at 60 dB SPL from the same ears. (C-D) ABR wave 1b thresholds presented at particular test frequencies (C) or wave 1b amplitude growth functions averaged across test frequencies (D). Inset: representative ABR waveforms from vehicle- and ouabain-treated ears evoked by 16kHz tone pips at 70dB SPL. Black arrowhead denotes wave 1b. All data are mean ± SEM.