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. 2019 Feb 28;9:3022. doi: 10.1038/s41598-019-39838-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of plant age on priming of ZAT10 and BAP1. Arabidopsis thaliana var. Col-0 were primed at an age of 2, 4 and 6 weeks by a 24 h cold-treatment at 4 °C. The 24 h 4 °C triggering stress was applied 5 days after priming. The transcript abundance for the primable ROS marker genes ZAT10 and BAP1 was evaluated directly after triggering in primed and triggered (PT), only primed (P), only triggered (T) and in control plants (C) and normalized to the geometric mean of the transcript levels of two constitutively expressed genes. As control for monitoring the cold-responsiveness, the transcript levels of the non-primable cold marker gene COR15A were determined. The letters refer to distinct significance groups as determined by ANOVA (Tukey’s test, p < 0.05, n = 3 ± SD).