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. 2019 Feb 28;9:3022. doi: 10.1038/s41598-019-39838-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Priming of leaves in different developmental stages of a 6-week-old rosette. Six week old plants were primed and triggered according to the experimental design (Fig. 7) and harvested after the triggering stimulus was applied. (A) The transcript abundance was measured for the primable ROS marker genes ZAT10 and BAP1 and normalized to the geometric mean of two constitutively expressed genes. Additionally the non-primable cold marker gene COR15A was analysed. (B) Transcript levels of the early senescence gene ORE1 were determined in the same samples as quantitative measure for the onset of senescence. An ANOVA (Tukey’s test, p < 0.05, n = 3 ± SD) was performed. The small letters refer to significance groups with leaf sets of the same age and different capital letters show significant differences between different age groups.