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. 2019 Feb 28;9:3022. doi: 10.1038/s41598-019-39838-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The effect of a prolonged lag-phase of 7 days on primable genes and tAPX expression. (A) Quantification of GUS activity in 4-week-old rosettes 5 (orange bar) or 7 (dotted bar) days after priming, respectively. The graph depicts the specific activity in primed plants at the end of the lag-phase relative to the specific activity in control plants (n = 10; mean ± SD, * t-Test p < 0.05. (B) Representative GUS staining pattern of tAPX_prom::GUS plants (n = 10) 5 or 7 days after cold-priming and in control plants. The arrows marks the leaf stage that was used for the quantification of GUS activity. (C) Comparison of the normalized tAPX transcript abundance 5 and 7 days after priming relative to the transcript levels in control plants (n = 3; mean ± SD, * one-sided t-Test p < 0.05). (D) Normalized transcript levels of ZAT10, BAP1, PAL1 and COR15A in PT-plants relative to T-plants at the time-point directly after the end of the triggering stimulus after a lag-phase length of either 5 days (green bar) or 7 days (dotted bar) (n = 3; mean ± SD, * one-sided t-Test p < 0.05).