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. 2019 Feb 28;9:3022. doi: 10.1038/s41598-019-39838-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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tAPX regulation in response to the priming stimulus. (A) The GUS activity in 2-, 4- and 6-week-old rosettes of primed tAPX_prom::GUS reporter gene plants 5 days after priming relative to the activity in untreated control plants of the same line (n = 10; mean ± SD, * t-Test p < 0.05). (B) GUS staining patterns of representative plants out of 10 individuals 5 days after priming (bottom) and un-treated controls (top) at different ages (2-, 4- and 6-week-old). The arrow indicates the developmental stage of leaves used for the GUS activity measurements. (B) tAPx transcript levels 0, 1 and 5 days (and 7 days additionally for 4-week-old plants) after priming relative to the levels of parallel cultivated untreated plants (n = 3; mean ± SD, * one-sided t-Test p < 0.05).