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. 2019 Feb 28;9:3044. doi: 10.1038/s41598-019-39269-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chemical structure and fluorescent distribution of an indocyanine green (ICG)-conjugated anti-cancer drug. (A) ICG is conjugated with gemcitabine (Gem) through coupling reactions, followed by deprotection. (B) Fluorescent and bright image (x100) of hepatocellular carcinoma (HCC) cells and colon cancer cell (HCT116), 24 h after exposure to ICG or ICG-Gem. Scale bar = 50 μm. The right lower panel shows the magnified fluorescent image of HCC cells 24 h after exposure to ICG-Gem.