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. 2019 Feb 28;9:2991. doi: 10.1038/s41598-019-39545-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aiolos overexpression increases metastasis in vivo. (A) Photographic pictures of the lungs of NOD-SCID mice 16 weeks after tail vein injection of H1299-Mock or H1299-Aiolos cells. White arrows indicated the metastatic nodules. (B) Quantification of the average numbers of metastatic foci in the lungs of mice. The asterisk (*) indicated statistical significance (P < 0.05) between experimental and control clones. (C) H&E staining of lung tissues of mice. Black arrows indicated the metastatic nodules. The photographs were taken at the magnification of x40.