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. 2019 Feb 28;9:2991. doi: 10.1038/s41598-019-39545-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Aiolos overexpression upregulates Twist and MMP16 expression. (A) Western blot analysis of Twist, Snail, Slug, and MMP16 expression in H1299-Aiolos vs H1299-Mock cells and in A549-Aiolos vs A549-Mock cells. Actin was used as a loading control. (B) Western blot analysis of Aiolos, Twist, E-cadherin, vimentin, and MMP16 in H1299-Aiolos-Scramble and H1299-Aiolos-Twisti clones and in A549-Aiolos-Scramble and A549-Aiolos-Twisti clones. Actin was used as a loading control. (C) Immuofluorescence staining of E-cadherin and vimentin in H1299-Aiolos-Scramble vs H1299-Aiolos-Twisti cells. The green and red signals represented the staining of E-cadherin and vimentin, respectively. The blue signal represented nuclear DNA staining by Hoechst 33342. (D) The number of H1299-Aiolos-Scrabmle vs H1299-Aiolos-Twisti cells that migrated across the membrane or invaded across the matrigel counted per high power field. Asterisk indicates P < 0.05, compared with control cells (Student’s t-test). (E) The number of A549-Aiolos-Scrabmle vs A549-Aiolos-Twisti cells that migrated across the membrane or invaded across the matrigel counted per high power field. Asterisk indicates P < 0.05, compared with control cells (Student’s t-test).