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. 2019 Feb 28;9:3161. doi: 10.1038/s41598-019-39341-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of structure alignment using image FFT and second order moments. (A) SEM of rSS-PCL scaffold; (B). MAP2 staining; drNPC in SPRPix matrix (rSS-PCL + PRP); (C). βIII-tubulin staining; drNPC in SPRPix matrix; (D). βIII-tubulin staining; drNPC in rSS-PCL scaffold; (E). βIII-tubulin staining; drNPC cultured on isotropic surface. Left – original grayscale images; Middle – FFT images with equivalent covariance ellipses; Right – angular distribution of FFT intensity with normal fit.