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. 2019 Feb 28;9:3161. doi: 10.1038/s41598-019-39341-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunofluorescence analysis of the spinal cord of a rhesus macaque at the site of implantation of the SPRPix matrix with human drNPC. (A) Representative low magnification image of the site of drNPC-SPRPix implantantation showing expression of human specific mitochondrial antigen (h-Mito) and βIII-tubulin (arrow) On the right: high magnification of the boxed area demonstrating co-localization of h-Mito and βIII-tubulin. (B) Rare example of colocalization of h-Mito with GFAP (arrow) at the site of transplantation. (C) Staining with human specific anti-Nestin and GFAP revealed numerous nestin-positive cells and the lack of astroglial reaction/differentiation. (D) Representative image of human specific anti-Nestin and MAP2. The white arrow points at the long MAP2-positive neurites derived from the human cells. The purple arrow shows the border of the spinal cord. (E) Control staining of naive spinal cord tissue demonstrated lack of h-Mito signal. Cell nuclei in all the images are stained with Hoechst (blue). Notations: rSS-PCL-spidroin-polycaprolactone scaffold; SC – spinal cord.