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. 2019 Feb 28;9:3129. doi: 10.1038/s41598-019-39960-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AAPP depletion prolongs probing and prediuresis time in TG mosquitoes. (A,B) Probing time (A) and prediuresis time (B) were determined in both WT and TG mosquitoes. Each dot represent a single female mosquito (n = 25). Mosquitoes with a probing time greater than 420 seconds were not included in statistical analysis. Results were analyzed by the Dunnett’s multiple comparisons test. Horizontal bars represent the median value. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.