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. 2019 Feb 28;9:3129. doi: 10.1038/s41598-019-39960-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Feeding success of TG mosquitoes on direct mouse feeding and artificial membrane feeding. (A) Feeding success of WT and TG mosquitoes both on naïve mice (Black bar) and artificial membrane feeding (Gray bar) for 10 min blood feeding. Percentage of successful feeding were analyzed by using a Chi-square test with 95% CI. (B,C) The size of blood meal ingested by mosquitoes that fed for 10 min on mice (B) or artificial membrane feeder (C) determined by the total hemoglobin contents in the abdomens of mosquitoes. (D,E) Fecundity rate (egg laying) of WT and TG mosquitoes both on mice (D) and artificial membrane feeding (E) for 10 min blood feeding. Results were analyzed by the Dunnett’s multiple comparisons test. Horizontal bars represent the median value. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. n.s., not significant.