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. 2019 Feb 28;9:3072. doi: 10.1038/s41598-018-35785-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Physical mapping of the telomeric repeats in the nuclei of three Karenia species at different stages of the asexual cycle. K. brevis (A,D,E), K. selliformis (B) and K. mikimotoi (C). Each panel shows merged images, to facilitate visualization of the DAPI-stained DNA (blue) and in situ hybridization of the Dy547-labeled oligonucleotide (CCCTAAA)₃ used to localize the telomeric repeats (red) during interphase (A,B) and mitotic stages (C–E). In D, the arrows indicate double visualized telomeric signals, and the arrowheads interstitial signals. The pairs of arrows in E indicate separate sister chromatids. ch = cytoplasmic channels. Scale bar = 10 μm for all panels.