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. 2019 Feb 28;9:3072. doi: 10.1038/s41598-018-35785-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Images of the AG-chromosome during the life cycle of Karenia mikimotoi. DAPI-stained DNA (blue) and in situ detection of the biotin-labeled (AG)₁₀ probe (red) in the painting of the AG-chromosome. Note the presence of a single AG-chromosome vs. a pair of AG-chromosomes in vegetative (haploids) (A–I) and planozygote (diploid) (J) cells, respectively. (A, E) nuclei in G2, (B) dividing nucleus, (D) a nucleus in S, (D) a putative gamete (F–I) in undergoing mitosis, (J) a zygote in G2. Scale bar = 10 μm.