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. 2019 Feb 28;9:3096. doi: 10.1038/s41598-019-39650-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Analysis of U87MG secretome from serum-containing (SCM) and serum-free media (SFM). (a) Schematic workflow for quantitative analysis of U87MG secretome between SCM and SFM. DDA: data-dependent acquisition. DDA-EL: DDA with exclusion list. (b) The number of identified proteins in the secretome. (c) The distribution of differentially secreted proteins between SFM and SCM. The H/M ratios are log2-transformed after normalization by the difference in growth rate. (d,e) Secretion pathways and subcellular localization of the differentially secreted proteins. Secretion pathways were analyzed by using SignalP, SecretomeP, and TMHMM (d), and subcellular localization by using Cello v2.5 (e).