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. 2019 Feb 28;9:3096. doi: 10.1038/s41598-019-39650-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of serum in media on proliferation of chondrocytes cocultured with hWJ-MSC. (a) Graphical representation of four different culture conditions. (b) Microscopic cell images of chondrocyte. (c) Cell proliferation assay performed by CCK-8 assay. Error bars are S.D. of triplicated experiments. (*p-value < 0.01). (d) Western blot images of cycinD1, SOX9, MMP3 and p53 proteins in chondrocytes. β-Actin was used as a control. Full-length images are shown in Supplementary Fig. S5a.