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. 2019 Feb 28;9:3160. doi: 10.1038/s41598-019-39220-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PRRSV immunofluorescence staining of PAMs infected with the Finistère strain at 7 h and 10 h post-infection (at MOI = 2) and controls. PRRSV indirect staining was performed with anti-PRRSV N protein antibody and anti-IgG Alexa 488-conjugated antibody (green). The nuclei were stained with Hoechst (blue). Magnification: 200X. Images are representative of two biological replicates with three technical replicates for each experimental condition. Viral titers are means ± standard deviations of two biological replicates with two technical replicates for each experimental condition.