Table 2.

Microarray (RIP-Chip experiment) and qPCR data of the expression of 8 genes in the whole cell and RISC compartments at 7 h and 10 h p.i.

Gene_Condition	Whole cell		RISC
	qPCR	array	qPCR	array
IFITM1_7 h p.i.	1.89	4.46	1,68	n.s.
IFITM1_10 h p.i.	1.45	5.16	2.66	n.s.
GBP1_7 h p.i.	1.84	1.87	1.47	2.45
GBP1_10 h p.i.	2.59	2.17	2.45	n.s.
ISG15_7 h p.i.	2.22	3.28	2.14	5.33
ISG15_10 h p.i.	1.93	5.64	0.50	n.s.
HMGB1_7 h p.i.	1.27	n.s.	0.78	0.31
HMGB1_10 h p.i.	0.78	0.51	1.79	1.42
OAS2_7 h p.i.	1.75	4.78	1.73	n.s.
OAS2_10 h p.i.	1.45	4.14	2.31	5.18
STAT1_7 h p.i.	1.55	2.11	1.07	1.68
STAT1_10 h p.i.	1.20	n.s.	1.93	n.s.
IFNB1_7 h p.i.	385	n.s.	105	n.s.
IFNB1_10 h p.i.	11,765	4.29	2,535	n.s.
FOXO4_7 h p.i.	0.88	n.s.	0.91	1.71
FOXO4_10 h p.i.	0.68	n.s.	0.96	n.s.

The RNAs for the qPCRs were obtained from PAMs from a second independent panel of 4 pigs (see Materials and Methods). The qPCR values were normalized vs. the geometric mean of GAPDH and B-Actin and calibrated vs. non-infected controls. All data are expressed as linear fold change values; “n.s.”: non significant after RIP-Chip analysis. The two-sided Spearman’s Rho test of the correlation of qPCR and microarray data was statistically significant, with r_s = 0.76 and p (2-tailed) <0.001.