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. 2019 Feb 28;9:3094. doi: 10.1038/s41598-019-39679-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A–D) Images of coronal brain sections showing DiI (red) labeled EVs taken up by IBA1 (blue) and CD11b (green) positive microglia. (E,F) Tomato and control plasmid (blue) dorsal SVZ electroporations stained for CD11b (green) and IBA1 (magenta) at P7, 10x (E), 20x (F). (G,H) 20x magnification of E showing CD11b (green) and IBA1 (red) (G) or CD11b (H). (I,J) Tomato and Rab27a shRNA plasmid (blue) dorsal SVZ electroporations stained for CD11b (green) and IBA1 (magenta) at P7, 10x (I), 20x (J). (K,L) 20x magnification of I showing CD11b (green) and IBA1 (red) (K) or CD11b (L). (M) Western blot of Rab27a following shRNA mediated knockdown. (N) Images of coronal section stained for Rab27a (green) following control or Rab27a shRNA and tomato (magenta) co-electroporation at P0 and sacrificed 48 hrs later. (O) Quantification of CD11b positive microglia at P2 and P7 following control or Rab27a shRNA electroporation. Data are represented as mean ± SEM. ****p < 0.0001 A, E, I scale bar, 100 μm. B–D, F–H, J–L, N scale bar, 50 μm.