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. 2019 Feb 28;9:3176. doi: 10.1038/s41598-019-39512-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deuterated distributions of the peptide ‘IAIKPDGVQRGLVGEIIKRFEQKGFRL’ from the oxidized protein at the same retention time with different D₂O labeling times. The distributions could not be fitted properly using a unimodal distribution, and deMix analyzed them as bimodal forms, whose theoretical distributions (shown as black circle) are well-fitted to their observed distributions. w represents relative amount of each component peptide species (shown as red rectangle and blue diamond).