Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 28;9:3099. doi: 10.1038/s41598-019-39430-9

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

The kAE1 E681Q mutant does not affect tight junction properties. mIMCD3 cells stably expressing kAE1 E681Q mutant were grown on semi-permeable filters for 10 days prior to mounting in Ussing chambers after inducing protein expression (+Dox). Measurements of TEER (A), P_Na/P_Cl ratio (B) and absolute permeability to sodium (C) or chloride (D) show that kAE1 E681Q mutant expression does not affect tight junction permeability, compared to kAE1 WT (mIMCD3 kAE1). Error bars correspond to means ± SEM, n = 7. No significant (n.s.) difference was observed in any of the conditions (One-way ANOVA) for mIMCD3 kAE1 E681Q cells, ****P < 0.0001 versus “mIMCD3 kAE1 - Dox” condition.