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. 2019 Feb 28;9:3099. doi: 10.1038/s41598-019-39430-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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kAE1 expression alters cytosolic pH and chloride concentration and pH of the basolateral growth medium. Chloride concentration (A) and pH (B) of the basolateral growth medium were measured on mIMCD3 kAE1 cells grown for 8 days on semi-permeable filters and kept un-induced or induced with doxycycline for 24 hours. (C) Initial cytosolic pH was measured for the 20 seconds before switching from a chloride-containing to a chloride-free Ringer’s perfusion solution in mIMCD3 kAE1 cells kept un-induced or induced for 24 hours with doxycycline. Error bars correspond to means ± SEM, n = 3–7, **P < 0.01, ***P < 0.001 and ****P < 0.0001 versus mIMCD3 kAE1 cells without doxycycline.