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. 2019 Feb 28;9:2990. doi: 10.1038/s41598-019-39643-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PTP4A3 expression reduces CRMP2 phosphorylation. (A) The phosphorylation state of CRMP2 was compared between OCM-1-EGFP-PTP4A3 and OCM1-EGFP-C104S cells by 2D gel electrophoresis. After Western blotting, detection was performed using an anti-CRMP2 antibody that recognizes total CRMP2 or phospho-specific anti-CRMP2 (S522) and anti-CRMP2 (T514) antibodies. (B) Spot densitometric profiles of phosphorylated CRMP2 were generated using ImageJ64. The grey peaks indicate those present for OCM1-EGFP-C104S cells which are absent from OCM1-EGFP-PTP4A3 cells. (C) Western blot analysis of total lysates (input) and lysates after phosphoprotein purification as loading controls.