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. 2019 Feb 28;9:2990. doi: 10.1038/s41598-019-39643-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CRMP2 affects the actin network. (A) Visualization of the actin cytoskeleton network in OCM-1 cells as assessed by Phalloïdin staining. After deconvolution, images were processed using ImageJ64. Scale bar = 20 μm. (B) Quantification of the number of fragments of < 40 adjoining pixels normalized to the cell area in OCM-1 cells. Data are shown as the mean values +/− SEM. ~20 cells were analyzed. ***p < 0.001, **p < 0.01, Student’s t-test. (C) Visualization of the actin cytoskeleton network in OCM-1 cells as assessed by Phalloïdin staining of cells treated with Y27632 for 24 h. After deconvolution, images were processed using ImageJ64. Scale bar = 20 μm. (D) Quantification of the number of fragments of < 40 adjoining pixels normalized to the cell area in OCM-1 cells treated or not with Y27632. Data are shown as the mean values +/− SEM. ~60 cells were analyzed. ***p < 0.001,*p < 0.01, *p < 0.05, Student’s t-test. (E) The mean overall speed of OCM-1 cells treated with Y27632 as assessed by a 2D random cell migration assayed by time-lapse video microscopy. Data are shown as the mean values +/− SD. ~100 cells were tracked per condition. ***p < 0.001, **p < 0.01, Student’s t- test.