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. 2019 Feb 28;9:3021. doi: 10.1038/s41598-019-39702-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal micrographs of the inner medulla of control (Cont) and K⁺-depleted (K⁺-Dep) Atg7^f/f and Atg7^Δpc mouse kidneys illustrating double labeling with pS261-AQP2 (red) and RAB9 (green). RAB9-positive puncta (green) is increased in both control and K⁺-Dep Atg7^Δpc mice; subsequently these changes in the K⁺-Dep Atg7^Δpc mice pronounced compared with Atg7^f/f mice. Note that pS261-AQP2 is not colocalized with RAB9-positive puncta (arrows) in the inner medullary collecting duct cells of K⁺-depleted Atg7^Δpc mice. Stars indicate the lumen of inner medullary collecting ducts.