Figure 4.

The activity of the MAPK Slt2 is essential for the increase in P-bodies under cell wall stress conditions. (a) Wild-type (WT) strain was transformed with the Dcp2-GFP plasmid. The slt2K54R strain corresponds to the slt2∆ mutant co-transformed with the Dcp2-GFP plasmid and a second plasmid expressing the kinase-death slt2K54R variant. Both strains were grown in the presence of 30 µg/ml CR or 0.8 U/ml ZY for one and four hours, and Dcp2-GFP foci were visualized by fluorescence microscopy. (b) P-bodies were visualized of in the WT and slt2∆ strains expressing Dcp2-GFP after growing in a medium containing 1 M KCl or 3 mM H₂O₂ for 15 min. The accompanying histograms reflect the quantitation of the microscopy data as described in Fig. 1. Statistical significance was determined using a two-tailed, unpaired, Student’s t test by comparing with the corresponding CR or ZY data from the wild-type strain (a) or no treatment conditions (b) (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001). Scale bar, 5 μm.