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. 2019 Feb 28;9:3186. doi: 10.1038/s41598-019-40112-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell wall stress induces localization of CWI-responsive mRNAs to P-bodies. (a) Confocal microscopy images of wild-type (WT) cells containing Dcp2-mCherry, coexpressing MLP1/CRG1/SRL3 or PGK1-U1A mRNA and U1A-GFP protein to allow analysis of mRNA localization growing in the absence or presence of 0.8 U/ml ZY for two hours, are represented. Control cells expressing only U1A-GFP protein in the absence of target mRNA are shown (upper panel). The colored overlay images show a 4.4 times enlargement of the image sections included in the squares and depict examples where the mRNAs colocalize with P-bodies (white arrowheads). (b) The graphs display the quantitation of the mRNA granules shown in (a) from three independent experiments (n > 100 cells). (c) Quantification of mRNA granules (GFP foci) in strains described in (a), treated with 3 mM H₂O₂ or starved for glucose for 15 min, is shown in the graphs. Statistical significance was determined using a two-tailed, unpaired, Student’s t test by comparing with no treatment conditions (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001; ns, not significant).