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. 2019 Feb 28;9:3095. doi: 10.1038/s41598-019-39543-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of DNA binding activities of XRCC1 truncations. (a) Domain organization of XRCC1 with truncation boundaries indicated by arrows: Full length (blue), 1–183 (purple), 219–633 (green), 219–415 (red), 219–300 (orange) and 301–415 (cyan). (b–g) DNA binding activity of XRCC1 truncations (µM concentrations) monitored by electrophoretic mobility shift using fluorescent 39 bp duplex DNA. Discontinuous PAGE was used to facilitate band resolution. Full length gels are in Supplementary Fig. 13.