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. 2019 Feb 28;10:975. doi: 10.1038/s41467-018-08267-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Hallmarks of ILCs by infant blood and intestinal CD45⁺lin⁻ cells. a viSNE plot of flow cytometric analysis of viable CD45⁺ cells showing separate clustering by tissue of origin: epithelium (EP), lamina propria (LP), and blood (B). b Individual representation of hallmarks of ILCs (CD56, CD16, KIR, CD127, CD94, CD7, NKp44, NKp46, NKp80, CD103, CD49a, and CD69) using viSNE algorithm of flow cytometric data of lin⁻(CD14⁻CD19⁻CD3⁻) cells for EP, LP, and blood samples. Expression is shown by color coding in relative intensity. viSNE plots have been calculated from concatenated FCS files gated on viable CD45⁺ lymphocytes from epithelium, lamina propria, and blood of matched donors (N = 4, iterations = 7500 perplexity = 100, KL divergence = 2.15)