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. 2019 Feb 28;9:3020. doi: 10.1038/s41598-019-39559-7

Figure 6.

Figure 6

LDL mimics the role of insulin in activation of glucose uptake through translocation of GLUT1. (A) HUVECs were cultured in glucose-free medium prior to experimentation and incubated with 2-NBDG (50 μM) for 30 min. Cells were then incubated with LDL (50 μg/mL, 30 min) or insulin (100 nM, 20 min), washed 3 times then lysates analyzed using a microplate reader and standardized with known protein concentrations. (B) HUVECs were starved by culture in serum-free medium then incubated with 50 μg/mL LDL for the indicated periods. Cytoplasmic and membrane proteins were extracted then analyzed by Western blotting and GLUT1 expression quantified. (C) HUVECs were starved by culture in serum-free medium and incubated with LDL (50 μg/mL, 30 min) or insulin (100 nM, 20 min). Cytoplasmic and membrane proteins were extracted and analyzed by western blotting and GLUT1 expression quantified. The expression of Ctr group cells was assigned the value of 1, n = 3, *p < 0.05, **p < 0.01 versus Ctr. #p < 0.05 versus LDL. Data expressed as mean ± S.E.M.