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. 2019 Feb 28;10:984. doi: 10.1038/s41467-019-08893-9

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Multivariate analysis of ER morphology and dynamics. Representative single optical sections of tobacco leaf epidermal cells transiently expressing GFP-HDEL (a, n = 46); after treatment with BFA (b, n = 46); Lat B (c, n = 28); heat shock (d, n = 33); or over-expression of RFP-LNP2 (e, n = 29), where n is the number of independent 5-frame time-series images. All scale bars = 5 µm. Pairwise scatter plots of the first 4 significant canonical variables from the MANOVA analysis grouped by treatment (f–h). The dotted line around each group represents the 95% confidence limit