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. 2019 Feb 28;9:3123. doi: 10.1038/s41598-019-39781-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) RP-HPLC elution profile of the products of the proteolysis of soluble 6aJL2 protein with trypsin. Prior to be injected into the column, the sample was reduced by adding 10 mM dithiothreitol (DTT). The data represented are the time-dependence variation of (black line) absorbance at 216 nm or (blue line) intrinsic fluorescence at 350 nm, exciting the sample at 295 nm. (B) Identification of the proteolytic fragments of 6aJL2 protein contained in the chromatography fractions collected in A) by MALDI-TOF mass spectrometry analysis. ^arefers to the fractions identified with the same letter code in panel (A). R.T./min refers to the retention time (minutes) of each fraction in the RP-HPLC analysis. ^brefers to the mass (Da) determined experimentally by MALDI-TOF mass spectrometry analysis. ^crefers to the segment of protein 6aJL2 identified as the best-matching sequence for each experimental mass determined by means of the web-based computational tool FindPept (http://web.expasy.org/findpept/).