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. 2019 Feb 28;9:3123. doi: 10.1038/s41598-019-39781-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Fibrillogenesis assay of the synthetic peptides with the sequence of the tryptic fragments of 6aJL2. Transmission electron micrographs of the aggregates formed by the synthetic peptides (B) Ser26-Arg39, (C) Ser26-Arg54, and (D) Phe62-Lys79 in the aggregation assay shown in panel (A). The images were obtained with a HITACHI-7100 transmission electron microscope. (E) Fibrillogenesis assay of the proteolytic fragment Thr18-Arg25-S-S-Thr80-Lys103 incubated in absence (T18-R25-S-S-T80-K103) or in presence of 10 mM DTT (T18-R25-S-S-T80-K103 + DTT). Fragment Thr18-Arg25-S-S-Thr80-Lys103 was generated by proteolysis of soluble 6aJL2 protein with trypsin and then purified by RP-HPLC, as described in Methods. Transmission electron micrographs of the aggregates formed by the tryptic fragment Thr18-Arg25-S-S-Thr80-Lys103 incubated in (F) absence or (G) in presence of 10 mM DTT, as shown in panel (E). The images were obtained with a CARL ZEISS Libra 120 transmission electron microscope. In (A) the data represented is the mean value ± S.D. of thioflavin T fluorescence emission at 482 nm of triplicate samples at the end of the experiment. In (E) the data represented is the mean ± 95 C.I. of the thioflavin T fluorescence emission at 482 nm of duplicated samples.