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. 2019 Feb 28;9:3166. doi: 10.1038/s41598-019-39405-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MALDI-MSI visualisation of TF3′G and ECG in jejunum membranes subjected to 60-min transport experiments in the absence or presence of influx transport inhibitors. Phloretin (200 µM), estrone-3-sulphate (100 µM), and wortmannin (1 µM) were used as influx transport inhibitors. TF3′G (m/z 715.1) and ECG (m/z 441.1), together with phloretin (m/z 273.1) and estrone-3-sulphate (m/z 349.1), were visualised via MALDI-MSI in the negative ion-linear mode at a spatial resolution of 50 µm using nifedipine/phytic acid as the matrix reagent. For visualisation of wortmannin (m/z 451.1), DHB (acetonitrile/water, 3/1, v/v, 20 mg/mL) was used as the matrix reagent in the positive ion-linear mode. Intensity signals corresponding to each of TF3′G, ECG, estrone-3-sulphate, and wortmannin are shown as fixed pseudocolour scales.