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. 2019 Feb 28;9:3166. doi: 10.1038/s41598-019-39405-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MALDI-MSI analyses of the metabolites TF3′G and ECG in jejunum membranes subjected to 60-min transport experiments in the presence of 20 µM cyclosporine A. TF3′G (m/z 715.1) and its metabolites [Me-TF3′G (m/z 729.1), sulphate-TF3′G (m/z 795.1), Me-sulphate-TF3′G (m/z 809.1), Glc A-TF3′G (m/z 891.2)] were visualised via MALDI-MSI in the negative ion-linear mode at a spatial resolution of 50 µm using nifedipine/phytic acid as the matrix reagent (a). ECG (m/z 441.1) and its metabolites [Me-ECG (m/z 455.1), sulphate-ECG (m/z 521.0), Me-sulphate-ECG (m/z 535.1), Glc A-ECG (m/z 617.1)] were visualised via MALDI-MSI in the negative ion-linear mode at a spatial resolution of 50 µm using nifedipine/phytic acid as the matrix reagent (d). Intensity signals corresponding to TF3′G, ECG, and their metabolites are shown as fixed pseudocolour scales. LC-TOF-MS chromatograms of TF3′G and its metabolites in the apical (b) and the basolateral solutions (c) were obtained after 60-min transport experiments using the SD rat jejunum membrane. LC-TOF-MS chromatograms of ECG and its metabolites in the apical (e) and the basolateral solutions (f) were obtained after 60-min transport experiments using the SD rat jejunum membrane. Detailed LC-TOF-MS conditions are described in the Methods section.