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. 2019 Feb 28;9:3037. doi: 10.1038/s41598-019-39403-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome-wide effects of eEF3 depletion on gene expression. The effects of eEF3 repression to ribosomal density (a), on the levels of mRNA (b) and ribosomal load (c) calculated for individual genes (2581 in total). Changes in gene coverage is considered significant when the Z-score of the differences is above 2 or below -2 in both replicates. Up-regulated genes in eEF3 depleted conditions are marked with red dots and down-regulated genes with blue dots, and the counts of genes presented as numbers in respective colors. Gene IDs of up-regulated and down-regulated genes, as well as associated GO IDs and p-values are provided in Supplementary Dataset 2. Read density is normalised as reads per kilobase per million (RPKM) of total reads for individual ORFs (a,b).