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. 2019 Feb 28;9:3037. doi: 10.1038/s41598-019-39403-y

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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eEF3 depletion causes moderate redistribution of the ribosome density along mRNAs. The graphs show distributions of polarity scores for individual ORFs (a,b, two experimental replicates), and average ribosome density in the vicinity of start and stop codons (c,d, two experimental replicates). The expression of eEF3 was either repressed by the addition of 0.5 mM methionine (red trace) or unrepressed (black trace, no methionine). The locations of start and stop codons are highlighted with green and blue backdrops, and the corresponding A-, P- and E-site positions of initiating and terminating ribosomes are indicated with letters under the x-axis.